Rate zonal sedimentation and isopycnic centrifugation
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چکیده
منابع مشابه
Separation of subcellular components and viruses by combined rate- and isopycnic-zonal centrifugation.
The re-isolation of trace anlOunts of virus added to tissue hOInogenates has been taken as a Inodel probleIll in the developIllent of high-resolution Illethods for separating particles. FroIll a review of data on these particles, it was found that, when sediIllentation rates are plotted against their banding or buoyant densities, IllOst viI'uses fall in a "window" which appears to be free of ot...
متن کاملIsolation of native glycogen by combined rate-zonal and isopycnic centrifugation.
Rat liver glycogen was separated into fractions of uniform particle size by the rate-zonal centrifugation of homogenates through cesium chloride and sucrose density gradients. Glycogen was then isolated from other cell constituents having similar sedimentation coefficients by isopycnic-zonal centrifugation in CsCI density gradients to effect a density separation. A density of 1.62 to 1.65 was i...
متن کاملIsopycnic centrifugation in ionic media.
Fritsch, A. (1973) Anal. Biochem. 35,57-71 Griffith, 0. M. (1978) Anal. Biochem. 9 0 , 4 3 5 4 4 3 Hames, B. D. & Rickwood, D. (1978) in Centr&gation-A Practical Approach (Rickwood, D., ed.), pp. 69-80, Information Retrieval. London Ridge, D. (1978) in Centrifugal Separations in Molecular and Cell Biology (Birnie, G. D. & Rickwood, D., eds.), pp. 33-61. Butterworths, London Young, B. D. (1978) ...
متن کاملResolution of Three Distinct Populations of Nerve Endings from Rat Brain Homogenates by Zonal Isopycnic Centrifugation
Conditions have been established for the fractionation of subcellular components of rat forebrain homogenates by zonal isopycnic equilibration in continuous sucrose density gradients using a B-XIV rotor. The fractions were analyzed biochemically and by ultra-structural morphometry. Starting from postnuclear supernates of forebrain homogenates, it has been possible to resolve three distinct popu...
متن کاملMaintenance of respiratory control in mitochondria after rate zonal centrifugation.
The respiratory control of rat liver mitochondria is lost when they are subjected to rate zonal centrifu~ation in a sucrose gradient (8.0% to 46.6%, w/w) at values for r t necessary for resolution. High sucrose concentrat ion and high ~ 2 t are bo th responsible. Respiratory control can be maintained in iso-osmotic Ficoll + 8.3% sucrose media, and after zonal centrifugation in such media at val...
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ژورنال
عنوان ژورنال: Journal of Chemical Education
سال: 1991
ISSN: 0021-9584,1938-1328
DOI: 10.1021/ed068p798